Fig. 2.

Degranulation and activation of dual-CAR NK-92. A CD107a expression demonstrated antigen-specific degranulation of CAR-engineered NK-92 during 5 h of co-culture at a 1:2 E:T ratio (N = 3). B High granzyme B secretion by dual-CAR NK-92 in the supernatant of 4 h co-cultures (1:1 E:T ratio; N = 3–6). C Dual-CAR NK-92 also significantly secrete IFN-γ upon activation (16 h co-culture at 1:1 E:T ratio; N = 3). Statistical analysis was performed using ANOVA with Dunnett’s correction for multiple comparisons with dual-CAR NK-92 as a reference. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. BCMA: B-cell maturation antigen; CAR: chimeric antigen receptor