Fig. 3.

The effect of irradiation on proliferation, viability, CAR expression and functionality of dual-CAR NK-92. A Proliferation of NK-92 cells (top) was successfully inhibited after irradiation and viability (bottom) gradually declined over the course of a week (N = 3). Follow-up on day 5 and 6 was not performed and, therefore, not shown in the graph. B Peak CAR expression (24 h post electroporation) is maintained following irradiation (N = 3). C Despite irradiation, cytotoxic activity of NK-92 towards Daudi, Namalwa and U266 cells was largely preserved (4 h co-culture at 1:1 E:T ratio). Statistical analysis in B and C between non-irradiated and irradiated conditions was performed using an unpaired, two-tailed student t test. *p < 0.05. BCMA: B-cell maturation antigen; CAR: chimeric antigen receptor