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. 2022 Mar 14;22:256. doi: 10.1186/s12879-021-06967-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Construction the Rv3737 knockout Mtb strain and Rv3737 Overexpression Msm strain. A The wildtype Rv3737 was substituted with DNA fragment encoding hygromycin resistance by homologous recombination. B Verification of the Rv3737 knockout Mtb strain by PCR amplification; M: DNA Marker 5000 bps; Lane 2: H37RvΔRv3737 left arm PCR product band; Lane 4: H37RvΔRv3737 right arm PCR product band; H37Rv-WT both arms PCR products have no bands. C Verification of the Rv3737 knockout Mtb strain by qPCR, t test, *p < 0.01. D Verification of the Rv3737 Overexpression Msm strain by Western blotting (full length gels and blots was provided in Additional file 1: Fig. S1 with changes marked)