Table 3.
Protease |
Molecular weights [kDa] |
Stability range |
Optimal conditions |
Common inhibitors |
||
---|---|---|---|---|---|---|
pH |
Temp. [°C] |
pH |
Temp. [°C] |
|||
Serine |
33–117 (majority 60–80) |
2.5–11 |
<80 |
5.2–10 |
40–75 (most of them 60–70) |
Diisopropyl fluorophosphate (DFP), Phenylmethanesulfonyl fluoride (PMSF), Pamidinomethanesulfonyl fluoride (APMSF), Chymostatin and Ciethyl pyrocarbonate (DEPC). |
Aspartic |
45–60 |
4.0–7.5 |
<70 |
4.5–6.5 |
60 |
Pepstatin A |
Cysteine |
21–29 |
3–12 |
<80 |
7–9 |
60 |
Iodoacetamide (IAA), p‐chloromercury benzoate (PCMB), sodium tetrathionate, mercuric chloride, transepoxysuccinyl‐ l‐leucylamido‐(4‐guanidino)butane (E‐64). |