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. 2022 Feb 28;13:849080. doi: 10.3389/fmicb.2022.849080

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Copyright © 2022 Miura, Nagata, Tamaki, Tomita and Kanai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogeny of the prokaryotic IEPs detected in this study. (A) An unrooted phylogenetic tree of representative IEP sets (1,949 proteins) in bacterial G2Is. The representative IEP sets were selected based on a similarity analysis (see section “Materials and Methods,” Prediction of IEP Sequences). The types of IEP are: A (bacterial-A, orange), B (bacterial-B, lavender), C (bacterial-C, yellow), D (bacterial-D, red), E (bacterial-E, light green), G [g1] (bacterial-G [g1], violet), F [g2–g5] (bacterial-F [g2–g5], plum), g6 (bacterial-g6, silver), ML (light yellow), CL1A (olive), CL1B (green), CL2A (blue), and CL2B (turquoise). U1–U3 (black) are newly identified clusters that were previously annotated as “unclassified.” (B) Distribution of the amino acid lengths of each canonical IEP. The peak relative density is set as 1.0 in each case.