Figure 2.

Lipid content of early or late-stage embryos with or without additives. (A) Flowchart depicting experimental design and generation of IVP embryos in experiment 2 part 1. (B) Quantification of Nile Red staining expressed by mean arbitrary fluorescent units (AFU, mean ± SEM). Lipid content was evaluated in early-stage embryos cultured with no additives (n = 37), early-stage embryos cultured with additives (n = 15), late-stage embryos with no additives (n = 26), and late-stage embryos with additives (n = 13). Values without common labels differ (P < 0.05).