Figure 3.

Effect of culture additives on embryonic mitochondrial polarity. (A) Flowchart depicting experimental design and generation of IVP embryos in experiment 2 part 2. (B) Embryos were cultured with given additives (l-carnitine, coenzyme Q [CoQ], Vitamin K₂ [VitK], and forskolin) and the mitochondrial polarity was assessed using Mitotracker Red staining. Mean mitochondrial polarity of embryos cultured in the presence of no additive (n = 9), l-carnitine (n = 9), CoQ (n = 8), VitK (10), forskolin (9), and a combination of all additives (n = 18) (mean AFU ± SEM, ^a,bP < 0.05).