Table 2.

Effect of different culture conditions on lipid accumulation

Media type	Interaction	n	Nile Red AFU	SEM
Continuous	None	149	33.78	± 0.90
3-Step Sequential	None	189	32.40	± 1.00
Continuous	Early (6)	80	39.34	± 1.17
3-Step Sequential	Early (6)	101	37.88	± 1.27
Continuous	Late (7)	69	28.21	± 1.30
3-Step Sequential	Late (7)	88	26.91	± 1.35
Continuous	Additive	112	29.38	± 0.90
3-Step Sequential	Additive	163	27.82	± 0.74
Continuous	No Additive	37	38.18	± 1.56
3-Step Sequential	No Additive	26	36.97	± 1.86

Embryos were cultured in designated treatment groups, then assessed for lipid content using Nile Red dye and quantified by mean arbitrary fluorescent units (AFU, mean ± SEM). There were no differences in the mean lipid content between the culture groups (P < 0.05).