Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Processing of a typical vertebral column to isolate vBA-MSCs. Vertebrae (typically T8 L5) were cleaned of soft tissue (A) before separating VBs and removing disks and remaining soft tissues (B). VBs were ground to approximately 1.5 cm3 fragments (C) before enzymatic digestion to release adherent cells. (D) Plastic-adherent vBA-MSCs form typical spindle shapes (passage 2 cells) in culture.
