Skip to main content
. Author manuscript; available in PMC: 2022 Mar 14.
Published in final edited form as: ACS Chem Neurosci. 2017 Dec 18;9(4):673–683. doi: 10.1021/acschemneuro.7b00263

Figure 3.

Figure 3.

Photophysical properties and optimal concentration of CX-G3. (a) Absorption spectrum of CX-G3 (10 μM) in aqueous solution at room temperature. (b) Fluorescence excitation spectrum (blue; λem = 480 nm) and emission spectrum (red; λex = 360 nm) of CX-G3 (10 μM) in aqueous solution at room temperature. (c) Fluorescence decay trace of CX-G3 in aqueous solution monitored at 480 nm with pulsed excitation at 335 nm (red). Instrument’s response function is in black, (d) Graph depicting pH-dependence of CX-G3 photon excitation and emission. (e) Sample images from serial dilution of CX-G3 in cultured cortical neurons in 96-well plate format. Concentration is indicated on the left. Size bar is 50 μm. (f) Graph of CX-G3 concentration vs intensity (expressed as fraction of G3 intensity at GFP-Syn puncta to average intensity over the entire plate). Higher values correspond to higher signal-to-noise ratio (values calculated from 3 wells/condition, confirmed by two independent dilution experiments). (g) Graph of CX-G3 concentration vs colocalization with GFP-Syn (values calculated from 3 wells/condition, confirmed by two independent dilution experiments).