TABLE 3.
Patient Characteristics and Genotyping Results Obtained Using the FastIDH Method and the Nextera XT Library Prep Kit
| Case No. | Age (y) | Sex | Side | Location | Subtype | WHO Grade | IDH Nextera Variant | MAF, % | IDH Fast Variant | MAF, % |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 53 | F | Right | Frontal | Anaplastic oligodendroglioma | III | IDH2 R172K | 56 | IDH2 R172K | 48 |
| 2 | 30 | M | Left | Frontal | Diffuse (fibrillar) astrocytoma | II | IDH2 R172M | 59 | IDH2 R172M | 46 |
| 3 | 32 | M | Left | Frontal | Diffuse (fibrillar) astrocytoma | II | IDH1 R132C | 14 | IDH1 R132C | 13 |
| 4 | 33 | F | Left | Frontal | Anaplastic astrocytoma | III | IDH1 R132C | 64 | IDH1 R132C | 48 |
| 5 | 33 | F | Left | Frontal | Diffuse (fibrillar) astrocytoma | II | IDH1 R132S | 35 | IDH1 R132S | 34 |
| 6 | 33 | F | Left | Frontal | Diffuse (fibrillar) astrocytoma | II | IDH1 R132H | 23 | IDH1 R132H | 29 |
| 7 | 35 | M | Left | Temporal | Diffuse astrocytoma | II | IDH1 R132H | 15 | IDH1 R132H | 35 |
| 8 | 38 | M | Left | Temporal | Diffuse (gemistocytar) astrocytoma | II | IDH1 R132H | 26 | IDH1 R132H | 28 |
| 9 | 54 | M | Left | Temporal | Anaplastic oligoastrocytoma | III | IDH2 R172K | 50 | IDH2 R172K | 38 |
| 10 | 47 | F | Right | Frontal | Diffuse astrocytoma | II | IDH1 R132H | 15 | IDH1 R132H | 15 |
| 11 | 45 | M | Right | Frontal | Glioblastoma | IV | Not analyzable | Not analyzable | ||
| 12 | 53 | M | Right | Temporal | Glioblastoma | IV | wt | Not analyzable |
Nucleotide substitutions in our samples for IDH mutations: IDH1 R132H-c.395G>A; IDH1 R132C-c.394C>T, IDH1 R132S-c.394C>A, IDH2 R172M-c.515G>T, IDH2 R172K-c.515G>A.
F indicates female; IDH, isocitrate dehydrogenase 1; M, male; MAF, mutation allelic fraction in the sample; wt, wild type.