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. 2021 Dec 29;54(4):566–581. doi: 10.1249/MSS.0000000000002841

FIGURE 2.

FIGURE 2

Aerobic voluntary wheel running rescues pain-induced occlusion of long-term potentiation in ACC slices. A–D, Typical immunohistochemistry images of c-Fos staining among the saline and sedentary group (A, Sal & Sed), the CFA and sedentary group (B, CFA & Sed), the saline and exercise group (C, Sal & Ex), and the CFA and exercise group (D, CFA & Ex). E, Statistical chart of c-Fos staining. n = 12 slice from four mice. F, G, Typical images of long-term potentiation (F) and electrode position (G) in the MED64 recording system. Lightning symbol, stimulation site; black line, fEPSP before TBS; red line, fEPSP after TBS; Roman numerals, ACC layers (F). H–L, Statistical chart of MED64 recordings of the fEPSP slope during the last 10 min (H). n = 6–7 slice. Typical images of MED64 recordings of fEPSP slopes among the saline and sedentary group (I, Sal & Sed), the CFA and sedentary group (J, CFA & Sed), the saline and exercise group (K, Sal & Ex), and the CFA and exercise group (L, CFA & Ex). Arrows, TBS (I–L). Kruskal-Wallis H test followed by Dunn’s multiple comparisons test was used to compare the Sal & Sed group with the CFA & Sed groups and the CFA & Sed group with the CFA & Ex group (E, ***P < 0.001, **P < 0.01). One-way ANOVA followed by Bonferroni’s multiple comparison test was performed between the Sal & Sed and CFA & Sed groups and the CFA & Sed and CFA & Ex groups (H, ***P < 0.001, **P < 0.01). The data are presented as the mean ± SEM.