TABLE 1.

Antimicrobial and cytotoxic effects of select bile salts and detergents

Bile salt or detergent	C. trachomatis, HeLa cells			HSV-2, primary cervical cells			HIV, MT-2 cells			ED50 for N. gonorrhoeae
	ED50a	CD50b	SI50c	ED50	CD50	SI50	ED50	CD50	SI50
NaC	>1	1.5	NDd	0.3	0.2	0.67	0.01	0.009	0.9	0.1
NaGC	>1	>5	ND	0.8	4	5	0.01	0.007	0.7	0.5
NaTLC-3-SO4	0.4	3.5	8.75	0.2	0.5	2.5	0.02	>0.1	>5	0.3
NaTC	>1	>5	ND	>1	3	ND	>0.1	>0.1	ND	0.4
N-9	0.03%	0.005%	0.17	0.003%	<0.001%	<0.3	NEe	NE	NE	0.02%
BZC	0.03	0.03	1	0.06	0.02	0.33	NE	NE	NE	<0.01

^aED₅₀, the dose of bile salt or detergent that inhibited 50% of chlamydial infection of HeLa cells, HSV-2 infection of primary cervical cells, HIV-1 infection of MT-2 cells, or gonococcal viability was determined from a dose-response curve generated from two to three independent experiments conducted at least in duplicate. Doses are in milligrams per milliliter for all compounds unless indicated otherwise. 

^bCD₅₀, the dose of bile salt or detergent that killed 50% of HeLa cells as measured with PI by flow cytometry analysis, primary cervical cells as measured by neutral red dye uptake, or MT-2 cells as measured by trypan blue exclusion. Doses are in milligrams per milliliter for all compounds unless indicated otherwise. 

^cSI₅₀ (selectivity index), ratio of CD₅₀ to ED₅₀. 

^dND, not determined. 

^eNE, not evaluated.