TABLE 2.

HSV-2 plaque formation following preincubation of virus or cells with bile salts

Compound (1 mg/ml)	PFU/dish (% of control)a
	Virusb	Cellsc	Virus-cellsd
NaC	20 ± 13	74 ± 21	3 ± 4
NaGC	59 ± 0.7	92 ± 13	60 ± 1.4
NaTLC-3-SO4	0e	96	0e

^aFor all three experimental methods, the results are presented as the number of PFU in the presence of compound as a percentage of the number of PFU in the presence of PBS. Each of the experiments was performed in duplicate with two different dilutions of virus. 

^bHSV-2(G) at a concentration of about 10⁹ PFU/ml was mixed with 1 mg of the indicated bile salt per ml or PBS as a control. After incubation for 1 h at 37°C, the mixtures were diluted 6 to 7 logs to yield noninhibitory levels of bile salt and 100 to 500 PFU/flask on control flasks and were plated on CaSki cells to determine whether the compound had irreversible effects on viral infectivity. Plaques were counted after 2 days in culture. 

^cFor the cytotoxicity assay, CaSki cells were incubated with 1 mg of the indicated bile salt per ml or PBS as a control for 1 h at 37°C and were then washed extensively prior to inoculation of the cells with virus in a standard plaque assay. 

^dBile salts were mixed with virus (100 to 500 PFU/ml) at a final concentration of 1 mg/ml immediately prior to inoculation of CaSki cells for a standard plaque assay. 

^eNo plaques were visualized on dishes inoculated with the same viral dilution.