Fig 2. Cells from 2DG-resistant strains 1–5 are resistant to 2DG, but the parental control and naïve control cells are not.

(A) Images of serial dilution growth assays on YPD (2% glucose as carbon source) or YPD with increasing concentrations of 2DG (0.05%, 0.2%, and 0.4%) after two days of growth at 30°C. (B) Graphs showing the change in cell density over time for cells grown in YPD or YPD with increasing 2DG concentrations, each of which have 2% glucose as a carbon source. Data are plotted as the A₆₀₀ measured every 30 minutes during a 24-hour time course. Curves represent the average A₆₀₀ of three experimental replicates, and vertical lines emerging from each data point represent standard deviations.