Table 1. Doubling times calculated from the growth curves shown in Fig 2B (hours).

	Parental ABC16-monster	Naïve ABC16-monster	2DG-resistant strain 1	2DG-resistant strain 2	2DG-resistant strain 3	2DG-resistant strain 4	2DG-resistant strain 5
YPD	2.58 ± 0.19 (ns)	2.48 ± 0.31 (ns)	2.35 ± 0.14	2.60 ± 0.32 (ns)	2.47 ± 0.29 (ns)	2.39 ± 0.22 (ns)	2.51 ± 0.20 (ns)
YPD + 0.05% 2DG	5.34 ± 0.43 (***)	5.61 ± 0.20 (***)	2.85 ± 0.22	2.86 ± 0.03 (ns)	2.75 ± 0.06 (ns)	2.65 ± 0.09 (ns)	2.84 ± 0.07 (ns)
YPD + 0.2% 2DG	ND	ND	3.18 ± 0.21	3.28 ± 0.05 (ns)	3.20 ± 0.06 (ns)	3.19 ± 0.1 (ns)	3.25 ± 0.09 (ns)
YPD + 0.4% 2DG	ND	ND	5.09 ± 0.61	4.6 ± 0.42 (ns)	4.59 ± 0.16 (ns)	5.2 ± 0.33 (ns)	4.91 ± 0.27 (ns)

Doubling times are presented in hours for the average of three replicate experiments, plus or minus the standard deviation. Statistical analyses using ANOVA to compare the doubling times of each sample in a specific condition to the doubling time of 2DG-resistant strain 1 are also provided in parenthesis, where a p-value of ns = not significant and

*** < 0.0001. None of the 2DG resistant strains are significantly different from one another under any growth condition. The parental and naïve ABC-monster strains are also not statistically different from one another or the 2DG resistant strains in YPD. However, the parental and naïve ABC-monster strains are statistically different from the 2DG resistant strains when grown in even the lowest 2DG concentration (0.05%).