Table 3. Yeast strains used in the current study.

Strain	Genotype	Source
BY4742	MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0	[128]
Parental ABC16-monster (RY0568)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0	[45]
Naïve ABC16-monster	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0	[45]
2DG Resistant Strain 1 (ABC16-monster)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0, hxk2G238V	This study.
2DG Resistant Strain 2 (ABC16-monster)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0, hxk2G238V	This study.
2DG Resistant Strain 3 (ABC16-monster)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0, hxk2G238V	This study.
2DG Resistant Strain 4 (ABC16-monster)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0, hxk2G238V	This study.
2DG Resistant Strain 5 (ABC16-monster)	MAT-α adp1Δ snq2Δ ycf1Δ pdr15Δ yor1Δ, vmr1Δ pdr11Δ, nft1Δ bpt1Δ ybt1Δ ynr070wΔ yol075cΔ aus1Δ pdr5Δ pdr10Δ pdr12Δ can1Δ::GMToolkit-α [CMVpr-rtTA NATMX4 STE3pr-LEU2] his3Δ1 leu2Δ0 ura3Δ0 met15Δ0, hxk2G238V	This study.
MSY1254	MAT-α ura3Δ0 leu2Δ0 his3Δ1 hxk2Δ::KANMX4	[40]
MSY1475	MAT-α ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 hxk1Δ::KANMX4 hxk2Δ::KANMX4 glk1Δ::KANMX4	[40]