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. 2022 Mar 2;18(3):e1009929. doi: 10.1371/journal.pcbi.1009929

Table 4. Plasmid DNA used in the current study.

Name Description Source
pRS313 CEN HIS3 [129]
pHxk2-3V5-313 Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; CEN HIS3 This study.
pRS315 CEN LEU2 [129]
pRS315-Hxk2-GFP Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to GFP; CEN HIS3 This study.
pRS315-Hxk2-G238V-GFP Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to GFP; the G238V mutation was introduced by site-directed mutagenesis; CEN HIS3 This study.
pRS315-Hxk2-3V5 Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; CEN HIS3 [40]
pRS315-Hxk2-G55V-3V5 Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; the G55V mutation was introduced by site-directed mutagenesis; CEN HIS3 [40]
pRS315-Hxk2-G238V-3V5 Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; the G238V mutation was introduced by site-directed mutagenesis; CEN HIS3 This study.