Table 4. Plasmid DNA used in the current study.
Name | Description | Source |
---|---|---|
pRS313 | CEN HIS3 | [129] |
pHxk2-3V5-313 | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; CEN HIS3 | This study. |
pRS315 | CEN LEU2 | [129] |
pRS315-Hxk2-GFP | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to GFP; CEN HIS3 | This study. |
pRS315-Hxk2-G238V-GFP | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to GFP; the G238V mutation was introduced by site-directed mutagenesis; CEN HIS3 | This study. |
pRS315-Hxk2-3V5 | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; CEN HIS3 | [40] |
pRS315-Hxk2-G55V-3V5 | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; the G55V mutation was introduced by site-directed mutagenesis; CEN HIS3 | [40] |
pRS315-Hxk2-G238V-3V5 | Genomic clone of HXK2 with 592 bp upstream of ATG and 373 bp downstream of the stop and a C-terminal fusion to 3V5; the G238V mutation was introduced by site-directed mutagenesis; CEN HIS3 | This study. |