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. 2021 Dec 30;17(1):2018857. doi: 10.1080/15592324.2021.2018857

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Analysis of chemical ER stress sensitivity to 0.75 mM DTT on fresh weight of indicated genotypes. Seedlings were grown vertically on solid MS media for 7 days, then transferred to fresh liquid MS media without (NT = no treatment) or with DTT. Seven days following DTT exposure, the seedlings were photographed (a) and total fresh weight of at least 30 plants per biological replication was recorded (b). At least three biological replications were performed. Statistical analyses were performed by two-tailed Student’s t-test or one-way ANOVA in Excel. Error bars show mean ± SD (n ≥ 30). Significant differences are indicated by asterisks (*** p < .001, ** p < .01), while “ns” indicates no statistically significant differences. Short solid bars connecting bars represent the comparison of fresh weight between untreated and treated samples for each genotype, while long solid lines represent the comparison of fresh weights of DTT-treated plants between Col-0 and an indicated mutant.