Figure 2.
Immunogenicity testing of neoantigens from patients with CRC. Autologous PBMCs were stimulated with candidate mutated peptides every 3 days in the presence of IL-2. On day 10, T-cell responses to each antigen were measured by an IFN-γ ELISPOT assay. The PBMCs in a, b, and c were obtained from PW4, PW10, and PW11 with CRC, respectively. No peptide (medium alone) or VSV-NP43-69 (STKVALNDLRAYVYQGIKSGNPSILHI) stimulation was used as a control. Data are presented as mean ± S.D. of three independent experiments. **p< .01 and *p< .05 compared with IFN-γ production by PBMCs stimulated without peptide or with VSV-NP43-69. NRTs, neoantigen-reactive T cells; SFC, spot-forming cell; VSV-NP, nucleoprotein of vesicular stomatitis virus; S.D., standard deviation.