Table 1. Fungal species used in the analysis with MbPOLIIF/R primers designed in the current study.
A positive reaction result distinguished by a visible band on the gel is indicated by a + sign, a negative response by a–sign. To confirm the presence of DNA, a control polymerase chain reaction (PCR) reaction with universal primers ITS1/ITS4 was performed on all samples. Reactions were always repeated three times.
| Species | Source | Code | Host | ITS1/4 | MbPOLIIF/R |
|---|---|---|---|---|---|
| Microdochium bolleyi | UPOC | UPOC-FUN-253 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium bolleyi | UPOC | UPOC-FUN-254 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium bolleyi | UPOC | UPOC-FUN-255 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium bolleyi | UPOC | UPOC-FUN-256 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium bolleyi | UPOC | UPOC-FUN-257 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium bolleyi | UPOC | UPOC-FUN-258 | Tritium aestivum | +/+/+ | +/+/+ |
| Microdochium nivale | AGT | 13M30 | Tritium aestivum | +/+/+ | -/-/- |
| Microdochium nivale | AGT | 17M323 | Tritium aestivum | +/+/+ | -/-/- |
| Microdochium nivale | AGT | 13M205 | Tritium aestivum | +/+/+ | -/-/- |
| Microdochium majus | AGT | 13M195 | Tritium aestivum | +/+/+ | -/-/- |
| Microdochium majus | AGT | 14M71 | Tritium aestivum | +/+/+ | -/-/- |
| Microdochium majus | AGT | 17M271 | Tritium aestivum | +/+/+ | -/-/- |
| Cochliobolus sativus | AGT | 07CS4.3 | Hordeum vulgare | +/+/+ | -/-/- |
| Ramularia collo-cygni | AGT | 20CZR19 | Hordeum vulgare | +/+/+ | -/-/- |
| Oculimacula yallundae | AGT | 15OY119 | Tritium aestivum | +/+/+ | -/-/- |
| Oculimacula acuformis | AGT | 15OA103 | Tritium aestivum | +/+/+ | -/-/- |
| Rhizoctonia cerealis | AGT | 20CC88 | Tritium aestivum | +/+/+ | -/-/- |
| Gaeumannomyces graminis var. tritici | CCM | F-575 | Tritium aestivum | +/+/+ | -/-/- |
| Rhynchosporium secalis | AGT | 18RhS04 | Hordeum vulgare | +/+/+ | -/-/- |
| Pyrenophora teres | AGT | 17PTT52 | Hordeum vulgare | +/+/+ | -/-/- |
| Pyrenophora maculata | AGT | 14PTM01 | Hordeum vulgare | +/+/+ | -/-/- |
| Pyrenophora tritici-repentis | AGT | 19DTR6 | Tritium aestivum | +/+/+ | -/-/- |
| Tilletia tritici | AGT | 06TCAR33 | Tritium aestivum | +/+/+ | -/-/- |
| Tilletia controversa | AGT | 06TCO02 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium graminearum | AGT | 20FG01 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium culmorum | AGT | 19FcBd | B. distachyon | +/+/+ | -/-/- |
| Fusarium avenaceum | CPPF | CPPF-161 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium poae | CPPF | CPPF-51 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium langsethiae | AGT | 12FL4.00 | Avena sativa | +/+/+ | -/-/- |
| Fusarium sporotrichioides | CPPF | CPPF-146 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium tricinctum | CPPF | CPPF-254 | Tritium aestivum | +/+/+ | -/-/- |
| Fusarium oxysporum | AGT | 19FOX06 | Zea mays | +/+/+ | -/-/- |
| Zymoseptoria tritici | AGT | ST-KM_B | Tritium aestivum | +/+/+ | -/-/- |
| Penicillium sp. | AGT | 20PEN_sp. | Hordeum vulgare | +/+/+ | -/-/- |
UPOC–Collection of Phytopathogenic Microorganisms, Czech Republic; AGT–Agrotest Fyto, Ltd, Czech Republic; CCM–Czech Collection of Microorganisms, Masaryk University, Faculty of Sciences, Czech Republic; CPPF—Collection of Phytopathogenic Fungi at Crop Research Institute Prague, Czech Republic.