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. 2022 Mar 15;132(6):e142137. doi: 10.1172/JCI142137

Figure 3. Eradication of GBM intracranial tumors, glial scar formation, and enhanced survival of mice induced by ADI-PEG20 in combination with ionizing radiation.

Figure 3

(A–C) Bioluminescence imaging (BLI) of intracranial tumors in mice using an IVIS Lumina II and Living Image software starting from day 13 after injection of GL261-Luc2 tumor cells. (D) Kaplan-Meier survival graph. Median survival times were 27, 47, and 37 days for saline, ADI-PEG20, and ionizing radiation (IR) monotherapy, respectively. Animals treated with combined treatment remained healthy and tumor free beyond 1 year. These animals received IR for 8 weeks and ADI-PEG20 for 13 weeks, after which time treatments were stopped. (E) Two animals from this group were culled on day 140 and brain sections were stained with H&E and for GFAP, showing evidence of histologically apparent glial scarring at the tumor site. Scale bars: 430 μm (left); 200 μm (middle, right). Data were analyzed using 2-way ANOVA with Tukey’s multiple comparison test, and adjusted P values are reported. ***P < 0.001 (saline vs. ADI-PEG20) + IR; §§§P < 0.001 (ADI-PEG20 vs. ADI-PEG20 + IR); ###P < 0.001 (IR vs. ADI-PEG20 + IR).