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. 2022 Mar 15;132(6):e142137. doi: 10.1172/JCI142137

Figure 4. Arginine deprivation increases recruitment of microglia into tumors and enhances their activity and phagocytic phenotype.

Figure 4

(A) Immunohistochemical evaluation of microglial/macrophage infiltration using Iba-1 staining on the contralateral nontumor side (green box), tumor edge (blue box), and intratumor (red box). (B) Intratumoral microglia occupancy. (C and D) Characterization of microglial phagocytic capacity using H&E and Oil red O staining of tumor sections and quantification of lipid bodies. (E and F) Assessment of the level of ˙NO production by microglia by 3-nitrotyrosine (3-NT) and Iba-1 staining of tumor sections. Iba-1 (green), 3-NT (red), colocalization of Iba-1 and 3-NT (yellow). Results were obtained from 5 animals per group. Note in the combined treatment group, analysis was carried out on the single animal showing evidence of tumor. Scale bars: 50 μm. Data were analyzed using 1-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.