Skip to main content
. 2022 Mar 15;132(6):e149828. doi: 10.1172/JCI149828

Figure 11. Skeletal muscle sex-specific enhancer landscape after weekly prednisone.

Figure 11

(A) C57BL/6J mice were treated for 4 weeks with vehicle or weekly prednisone and then analyzed 4, 6, or 8 days after the final injection. Arrows indicate i.p. injections; bars indicate no injection. (B and C) Enhancers present 4 days after the final prednisone injection but not present in vehicle-treated myofibers were mostly unique to one sex (B) and predominantly intergenic or intronic (C). TTS, transcription termination site. (D) Percentage of enhancers present 4 days after final prednisone injection that were still present after 6 and 8 days. (E–G) ChIP-Seq tracks showing presence (E) or absence (F and G) of H3K27ac enrichment at putative GREs (gray boxes). (H) ChIP-Seq tracks showing female-specific enhancer peaks (gray boxes) near Esr1.