Skip to main content
. 2022 Mar 15;132(6):e149828. doi: 10.1172/JCI149828

Figure 3. Weekly prednisone elicits distinct transcriptional response in male versus female muscle.

Figure 3

(A) Transcriptome comparison of once-weekly prednisoneā€“treated muscle to vehicle-treated for both sexes; prednisone-responsive genes were identified as being above expression and fold-change (FC) thresholds and below a variation threshold. n = 3 animals per group. (B) The majority of prednisone-responsive upregulated genes were unique to one sex. (C) Gene ontology analysis of male- and female-only upregulated genes shows differential pathway enrichment. (D and E) Pheno-RNA analysis of genes shows high correlation between gene expression and physiological response in males (D) and females (E). Genes responsive to weekly prednisone are more correlated with physiological response than a shuffled control group and genes in pathways of interest are more correlated with physiological response than prednisone-responsive genes involved in other cellular processes.