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. 2022 Mar 9;60(1):589–599. doi: 10.1080/13880209.2022.2039722

Figure 1.

Figure 1.

EGCG inhibited the levels of miR-29b-3p, MMP-13 and IL-6β but promoted cell viability on IL-1β-stimulated CHON-001 cells. (A) MiR-29b-3p expression in OA tissues and normal tissue was detected by qRT-PCR. (B) HE and Safranin O staining were used to detect the pathological changes of cartilage tissue in OA patients and healthy people. (C) The level of miR-29b-3p was determined by qRT-PCR in CHON-001 cells. (D) Cell counting kit-8 (CCK-8) indicated cell viability in CHON-001 cells. (E, F) The levels of MMP-13 and IL-6 were determined by ELISA in CHON-001 cells. U6 was used as a reference gene for miRNA. EGCG: epigallocatechin-3-O-gallate; EGCG20: 20 μM EGCG; EGCG50: 50 μM EGCG; qRT-PCR: real-time reverse transcription polymerase chain reaction; ELISA: enzyme-linked immunosorbent assay; control: CHON-001 cells cultured without IL-1β or EGCG. ΔΔΔp < 0.001 vs. control; ∧p < 0.05 or ∧∧p < 0.01 or ∧∧∧p < 0.001 vs. IL-1β. All measurements were performed at least three times. Data were presented as the means ± standard deviation (SD).