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. 2022 Mar 9;60(1):589–599. doi: 10.1080/13880209.2022.2039722

Figure 4.

Figure 4.

Overexpressed miR-29b-3p affected viability and the levels of MMP-13 and IL-6 of IL-1β-stimulated CHON-001 cells via down-regulating PTEN. (A–F) CHON-001 cells were divided into NC, PTEN, M, M + IL-1β, M + PTEN and M + IL-1β + PTEN groups. The protein (A, B) and mRNA (C) levels of PTEN in CHON-001 cells were determined by Western blot and qRT-PCR, respectively. (D) Cell counting kit-8 (CCK-8) assay indicated viability of CHON-001 cells. (E, F) The levels of MMP-13 and IL-6 were determined by ELISA in CHON-001 cells. GAPDH was used as the internal reference control for PTEN. MC: mimics control; M: miR-29b-3p mimics; qRT-PCR: real-time reverse transcription polymerase chain reaction; ELISA: enzyme-linked immunosorbent assay. *p < 0.05 or **p < 0.01 or ***p < 0.001 vs. NC; ∧∧∧p < 0.001 vs. M + IL-1β; ΔΔΔp < 0.001 vs. M + PTEN. All measurements were performed at least three times. Data were presented as the means ± standard deviation (SD).