FIGURE 1.

Loss of Sirt6 causes accelerated larval development and adult hypertrophy in Drosophila. (a) Larval body size at 24, 48 and 72 h post‐synchronised egg laying. w¹¹¹⁸ (control) and Sirt6^{−/−bck−L1} larvae were imaged at 12.5× magnification. (b) Percentage pupation in backcrossed Sirt1^{−/− bck} , Sirt4^{−/− bck} 4 and Sirt6^{−/−bck−L1}  larvae compared to w¹¹¹⁸ flies (N = 3, n = 150–200). (c) Representative image of 3–5 day old w¹¹¹⁸ and Sirt6^{−/−bck−L1/2} flies. (d) Body weight measurement in w¹¹¹⁸ and Sirt6^{−/−bck−L1/2} flies (N = 3, n = 20–25 per genotype). (e) Relative wing dimensions in w¹¹¹⁸ and Sirt6^{−/−bck−L1/2} flies (N = 3, n = 20–25). (f) Wing aspect ratio in w¹¹¹⁸ and Sirt6^{−/−bck−L1/2} flies, as computed from (e). (g) Larval body size at 24, 48 and 72 h post‐synchronised egg laying in the following genotypes: 1‐Sirt6^OE , 2‐actingal4, 3‐Sirt6^OE ; Sirt6^{−/−bck−L1} , 4‐actingal4; Sirt6^{−/−bck−L1} and 5‐actingal4/Sirt6^OE ; Sirt6^{−/−bck−L1} . (h‐k) Genetic rescue of Sirt6 restores early pupation (H), body weight (I), wing dimensions (J) and wing aspect ratio (k) phenotype (N = 3, n = 20–25 per genotype). All data presented are mean ± SEM Asterisk depicts p values (*p < 0.05, **p < 0.01 and ***p < 0.001) as observed by Student's t‐test and two‐way ANOVA, as applicable