Figure 7.

Effects of GANT61, cyclopamine, RU-SKI43 and sunitinib on (A-C) GLI1, (D-F) PTCH1, (G-I) BCL2, (J-L) MYC, (M-O) VEGFA and (P-R) CCND1 mRNA expression in 786-O, ACHN and HK2 cells. The cells were treated with each compound at the EC₅₀ and EC₂₅ for 48 h. cDNA was obtained from cells by RNA isolation and further reverse transcription. Gene expression was measured by qPCR. Statistical significance was estimated using one-way ANOVA. Data are presented as the mean ± SEM of three independent experiments carried out in duplicate. ^*P<0.05, ^**P<0.01, ^***P<0.001, ^****P<0.0001 vs. control. GLI1, glioma-associated zinc finger protein 1; PTCH1, patched 1; CCND1, cyclin D1; VEGFA, vascular endothelial growth factor A; qPCR, quantitative PCR; EC₂₅, 25% effective concentration; EC₅₀, half maximal effective concentration.