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Map of pPON-1 insert from M. morganii 1 and the primers used to PCR amplify the indicated genes from 15 M. morganii clinical isolates: primers 1 and 4 for hybF, ampR, and ampC; primers 2 and 4 for ampR and ampC; primers 3 and 4 for ampC; and primers 3 and 5 for ampC and orf-1. Cloning of ampR-ampC genes or of the ampC gene into pACYC184, giving recombinant plasmids pAC-1 or pAC-2, respectively, used similarly PCR-amplified fragments.
