Fluorescence lifetime of rose Bengal and Rhodamin B mixture solution changes as a function of the concentration of rose Bengal. In the mixture solutions, rose Bengal concentrations of 0, 0.25, 0.50, 0.75, and 1.0 μM also correlate to Rhodamin B concentrations of 1.0, 0.75, 0.50, 0.25, and 0 μM, respectively. Lifetime was deconvoluted based on the Laguerre basis expansion technique.