Table 1. List of key oligonucleotides for CRISPR.
Name | Sequence | Source |
---|---|---|
crRNA(20 bp immediately 5' to the PAM sequence) | ||
casy-1(wp78) 5' | gtgagggtggaaaatgattg | IDT |
casy-1(wp78) 3' | tgtgatgtaatcaacagggt | IDT |
unc-43-gfp11x7 | gagaaaaataggcataaaga | IDT |
casy-1-gfp11x7#1 | agaacgagcgttcgttgaga | SunyBiotech |
casy-1-gfp11x7#2 | tgtcgttggaggtcttgagt | SunyBiotech |
lin-10-gfp11x7 | ataaacaatcaaatgtattg | IDT |
Genotyping primers | ||
casy-1(wp78) | f: gaataagaatgagaagacccgctgc; r1: ctccttgcagattgattattggcgc; r2: aaggagtgaaaaggacagtatgaagacg | IDT |
unc-43-gfp11x7 | f: tcagaaacggagaagctcatacccg; r1: tcatgtagtaccatatggtcgcgtcc; r2: ccatatatctgagagaatgggacaag | IDT |
casy-1-gfp11x7 | f: aaattccttcaggcatgttg; r: gaaggagtgaaaaggacagt | IDT |
lin-10-gfp11x7 | f: tcgcagttgcacatgacaggtgag; r: attcacattagggcgcactttctgg | IDT |
Others | ||
7XGFP11 template | tcaggaggccgtgaccacatggtccttcatgagtatgtaaa tgctgctgggattacaggtggctctggaggtagagatcatat ggttctccacgaatacgttaacgccgcaggcatcactggcgg tagtggaggacgcgaccatatggtactacatgaatatgtcaatg cagccggaataaccggagggtccggaggccgggatcacat ggtgctgcatgagtatgtgaacgcggcgggtataactggtggg tcgggcggacgagaccatatggtgcttcacgaatacgtaaacg cagctggcattactggcggatcaggtggcagggatcacatggt actccatgagtacgtgaacgctgctggaatcacaggcggtagcgg cggtcgggaccatatggtcctgcacgaatatgtcaatgctg ccggtatcaccggcggcaag |
IDT |
GFP1-10 template | atgtccaaaggagaagaactgtttacgggtgttgtgccaatttt ggttgaactcgatggtgatgtcaacggacataagttctcagtg agaggcgaaggagaaggtgacgccaccattggaaaattg actcttaaattcatctgtactactggtaaacttcctgtaccatgg ccgactctcgtaacaacgcttacgtacggagttcagtgcttttc gagatacccagaccatatgaaaagacatgacttttttaagtc ggctatgcctgaaggttacgtgcaagaaagaacaatttcgttc aaagatgatggaaaatataaaactagagcagttgttaaattt gaaggagatactttggttaaccgcattgaactgaaaggaacag attttaaagaagatggtaatattcttggacacaaactcgaatacaa ttttaatagtcataacgtatacatcactgctgataagcaaaagaac ggaattaaagcgaatttcacagtacgccataatgtagaagatgg cagtgttcaacttgccgaccattaccaacaaaacacccctattgg agacggtccggtacttcttcctgataatcactacctctcaacacaaa cagtcctgagcaaagatccaaatgaaaaataa |
IDT |