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. 2022 Mar 14;11:e73557. doi: 10.7554/eLife.73557

Table 1. List of key oligonucleotides for CRISPR.

Name Sequence Source
crRNA(20 bp immediately 5' to the PAM sequence)
casy-1(wp78) 5' gtgagggtggaaaatgattg IDT
casy-1(wp78) 3' tgtgatgtaatcaacagggt IDT
unc-43-gfp11x7 gagaaaaataggcataaaga IDT
casy-1-gfp11x7#1 agaacgagcgttcgttgaga SunyBiotech
casy-1-gfp11x7#2 tgtcgttggaggtcttgagt SunyBiotech
lin-10-gfp11x7 ataaacaatcaaatgtattg IDT
Genotyping primers
casy-1(wp78) f: gaataagaatgagaagacccgctgc; r1: ctccttgcagattgattattggcgc; r2: aaggagtgaaaaggacagtatgaagacg IDT
unc-43-gfp11x7 f: tcagaaacggagaagctcatacccg; r1: tcatgtagtaccatatggtcgcgtcc; r2: ccatatatctgagagaatgggacaag IDT
casy-1-gfp11x7 f: aaattccttcaggcatgttg; r: gaaggagtgaaaaggacagt IDT
lin-10-gfp11x7 f: tcgcagttgcacatgacaggtgag; r: attcacattagggcgcactttctgg IDT
Others
7XGFP11 template tcaggaggccgtgaccacatggtccttcatgagtatgtaaa
tgctgctgggattacaggtggctctggaggtagagatcatat
ggttctccacgaatacgttaacgccgcaggcatcactggcgg
tagtggaggacgcgaccatatggtactacatgaatatgtcaatg
cagccggaataaccggagggtccggaggccgggatcacat
ggtgctgcatgagtatgtgaacgcggcgggtataactggtggg
tcgggcggacgagaccatatggtgcttcacgaatacgtaaacg
cagctggcattactggcggatcaggtggcagggatcacatggt
actccatgagtacgtgaacgctgctggaatcacaggcggtagcgg
cggtcgggaccatatggtcctgcacgaatatgtcaatgctg
ccggtatcaccggcggcaag
IDT
GFP1-10 template atgtccaaaggagaagaactgtttacgggtgttgtgccaatttt
ggttgaactcgatggtgatgtcaacggacataagttctcagtg
agaggcgaaggagaaggtgacgccaccattggaaaattg
actcttaaattcatctgtactactggtaaacttcctgtaccatgg
ccgactctcgtaacaacgcttacgtacggagttcagtgcttttc
gagatacccagaccatatgaaaagacatgacttttttaagtc
ggctatgcctgaaggttacgtgcaagaaagaacaatttcgttc
aaagatgatggaaaatataaaactagagcagttgttaaattt
gaaggagatactttggttaaccgcattgaactgaaaggaacag
attttaaagaagatggtaatattcttggacacaaactcgaatacaa
ttttaatagtcataacgtatacatcactgctgataagcaaaagaac
ggaattaaagcgaatttcacagtacgccataatgtagaagatgg
cagtgttcaacttgccgaccattaccaacaaaacacccctattgg
agacggtccggtacttcttcctgataatcactacctctcaacacaaa
cagtcctgagcaaagatccaaatgaaaaataa
IDT