Table 3.

Ct value comparison for direct and matrix pool testing.

Ct value range for individual positive sample	Ct (Mean±SD)
	Direct	5 × 5	10 × 10
		200 µL	200 µL	400 µL
≤ 25	23.3 ± 0.9	28.8 ± 1.8	30.4 ± 0.9	30.2 ± 1.3
26–30	27.8 ± 1.7	29.4 ± 3.0	32.6 ± 2.3	32.2 ± 2.6
31–35	31.2 ± 0.8	31.9 ± 3.4	33.2 ± 1.6	32.8 ± 1.9
Overall	27.43 ± 3.4	30.03 ± 2.73	32.06 ± 1.6	31.73 + 1.93