Figure 4.

Upregulated circPSD3 inhibits ccRCC metastasis by mediating EMT in vivo. (a) Schematic of the lung metastasis model was established in BALB/C nude mice with the indicated cells. (b) Bioluminescence imaging and quantification of lung metastasis after injecting tumor cells into the tail veins of nude mice (N = 5). (c) Representative images of lung metastatic tumors were obtained from an in vivo lung metastasis model established via tail vein injection of tumor cells. The right panel shows the counts of the lung metastatic nodules (N = 5). (d) Representative images of H&E and IHC staining of E-cadherin, N-cadherin, and vimentin in xenograft tumors. Scale bar, 100 μm. The data are presented as mean ± SD based on triplicate independent experiments.  ^∗ ∗P < 0.01;  ^{∗ ∗ ∗}P < 0.001.