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. 2022 Mar 7;2022:5084631. doi: 10.1155/2022/5084631

Figure 5.

Figure 5

circPSD3 is a sponge for miR-25-3p. (a) Six candidate target miRNAs of circPSD3 were simultaneously predicted by miRanda and starBase software. (b, c) The circRNA pull-down experiments were performed with Caki-1 cells using a circPSD3-specific probe. The enrichment of circPSD3 and the 6 miRNAs was detected by qRT-PCR and normalized to the levels detected by a control probe. (d) The luciferase reporter assay was performed to detect the activity of luc-circPSD3 and luc-circPSD3 mutant in 293T cells after the transfection of miR-25-3p mimics. (e) RNA FISH immunofluorescence shows the colocalization of circPSD3 (red) with miR-25-3p (green) in Caki-1 cells. Scale bar, 50 μm. (f) qRT-PCR was performed to detect miR-25-3p expression in adjacent nontumor and ccRCC tissues. (g) The circPSD3 expression was negatively correlated with miR-25-3p expression in our patient cohort (n = 81). The data are presented as mean ± SD.  ∗ ∗ ∗P < 0.001; NS, no significance.