TABLE 4.

Effect of sera from different animals on antibacterial activity of pexiganan^a

Organism	MIC (μg/ml) in 50% serum from the following animal + 50% MHB:						MIC (μg/ml) in 100% MHB
	Mouse	Rat	Rabbit	Swine	Bovine	Human
Staphylococcus aureus ATCC 25923	>256	16	16	8	128	8	4
Escherichia coli ATCC 25922	128	256	16	32	128	16	8
Pseudomonas aeruginosa ATCC 27853	>256	>256	128	32	32	32	16

^aThe protocol used for evaluation of the inhibition of antimicrobial activity of pexiganan by serum was a variation of the NCCLS broth microdilution assay (15) with a standard (100 μl) volume containing 50 μl of serum and 50 μl of unsupplemented MHB. The results were compared to those for growth controls without serum. The serum samples used were not heat inactivated.