Table 1. Characterization of blaNDM-5-carrying Escherichia coli andCitrobacter freundii isolates .
| Isolates | Species | MLST | Farmers market (FM) | Other resistance genes | Heavy metal-resistant genes | Chromosomal mutations | Location of blaNDM-5 | Plasmid
size (bp) |
Conjugation frequencya |
| a: Average±Standard error (SE). N.D.: Not detected. Bold: blaNDM-5−carrying plasmids and other resistance genes. | |||||||||
| BY9F33M | E. coli | ST57 | FM4 | aac(3)-IV, aadA2b, aph(3'')-Ib, aph(3')-Ia, aph(6)-Id, aph(4)-Ia, blaTEM-1B, floR, sul3, cmlA1 aadA1 , aadA5, aph(3')-IIa, mdf(A), mph(A), qnrS1, arr-2, sul1, tet(A), dfrA14, dfrA27 | terDZW,merPRT, arsADR | GyrA (p.S83L, p.D87N)
ParC (p.S80I) |
IncHI2, IncFIB, IncX1, IncY | 238 926 | (4.93±0.91)×10−6 |
| BY9F36M | C. freundii | ST557 | FM4 | aac(3)-IId, aadA16,aac(6')-Ib-cr, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, blaCMY-129, mph(A), floR, qnrB18, qnrB26, qnrB6, arr-3, sul1, sul2, tet(A), dfrA27 | arsADR, merCPTR | N.D. | IncX3, IncFIB (K) | 46 161 | (2.05±0.21)×10−4 |
| PY9F04M | E. coli | ST48 | FM21 | aadA1,aadA2, aph(3')-Ia, blaOXA-10, mdf(A), mph(A), erm(42), cmlA1, floR, oqxAB, arr-2, sul2, tet(A), tet(M), dfrA12, dfrA14 | pcoABCDESR,iucABCD, silABCEFPRS | GyrA (p.S83L, p.D87N)
ParC (p.S80I) |
IncX3, IncFII, IncFIB, IncFIA | 46 161 | (3.75±0.80)×10−5 |
| PY9F07M | E. coli | ST48 | FM21 | ant(3'')-Ia, aadA1,aadA2,aph(3')-Ia, blaOXA-10, mdf(A), mph(A), erm(42), cmlA1, floR, oqxAB, arr-2, sul2, tet(A), tet(M), dfrA12, dfrA14 | pcoABCDESR,iucABCD, silABCEFPRS | GyrA (p.S83L, p.D87N)
ParC (p.S80I) |
IncX3, IncFII, IncFIB, IncFIA | 46 161 | (2.88±1.41)×10−5 |
| PY9F09M | E. coli | ST155 | FM21 | aadA1,blaOXA-10, mdf(A), cmlA1, floR, qnrS1, arr-2, tet(A), dfrA14 | − | N.D. | IncX3, IncFIB | 46 161 | (4.87±0.25)×10−5 |
| TH9F11M | E. coli | ST101 | FM8 | aac(3)-IV, aadA2b, aph(3'')-Ib, aph(3')-Ia, aph(6)-Id, aph(4)-Ia, blaOXA-10,blaCTX-M-55, fosA3, cmlA1, floR, qnrS1, sul3, tet(A), dfrA1, dfrA14, arr-3,mdf(A), arr-2, sul2 |
iroBCDEN,
arsADR, terDZW |
GyrA (p.S83L, p.D87N)
ParC (p.S80I) ParE (p.S458A) |
IncHI2-IncF, IncFII, p0111 | 407 456 | (2.79±0.35)×10−5 |
| HZ9F01M | E. coli | ST9124 | FM12 | aadA22, aph(3')-Ia, aph(6)-Id, blaTEM-1B, mdf(A), mph(A), lnu(F), floR, qnrS1, arr-2, sul3, tet(A), dfrA14 | terDZW | GyrA (p.S83L, p.D87N)
ParC (p.S80I) |
IncX3, IncFII, IncHI2, IncI1 | 46 161 | (2.35±0.31)×10−5 |