Figure 6.

Application of CAR-Treg cells in animal model of MS

Chronic EAE mouse model is used in MS, induced by MOG_35-55 and enhanced by Freund’s adjuvant (CFA) or pertussis toxin (PT) (Berard et al., 2010). CARαMOG-FoxP3-Tregs express MOG-targeting CAR, FoxP3, and CD28-CD3ζ signaling domains (Fransson et al., 2012). From in vitro experiments, CARαMOG-FoxP3-Tregs inhibit T cell proliferation in the presence of MOG⁺ cells. Based on the bystander effect, both MBP-CAR Treg and MOG-CAR Treg therapies have been used for EAE model treatment, with their combination significantly inhibiting inflammatory and myelin-specific T cell responses, and thereby EAE progress. EAE: Experimental autoimmune encephalomyelitis; MBP: Myelin basic protein; MOG: Myelin oligodendrocyte glycoprotein; scFv: Single chain antibody fragment; CTL: Cytotoxic T lymphocyte.