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. 2022 Mar 18;43(2):285–300. doi: 10.24272/j.issn.2095-8137.2021.460

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Editorial Office of Zoological Research, Kunming Institute of Zoology, Chinese Academy of Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Vibrio splendidus infection and LPS challenge induced mitochondrial damage and mitophagy in coelomocytes

A: Representative TEM images of morphology of damaged mitochondria (arrowhead) in coelomocytes of A. japonicus infected by V. splendidus for 48 h. B: Representative TEM images of autophagosomes engulfing mitochondria (arrowhead) in coelomocytes of A. japonicus infected by V. splendidus for 48 h. C: ΔΨm of coelomocytes after LPS treatment for 0, 1, 3, 6, 12, and 24 h determined by JC-1 staining and flow cytometry. ^*: P<0.05;^**: P<0.01,n=3. D: Immunofluorescence colocalization analysis of Lyso-Tracker Red with Mito-Tracker Green in coelomocytes after LPS exposure for 0, 1, 3, 6, 12, and 24 h, with DAPI (blue) staining to identify nuclei. M: Mitochondria.