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. 2022 Mar 18;43(2):285–300. doi: 10.24272/j.issn.2095-8137.2021.460

Figure 5.

Figure 5

AjBNIP3-mediated mitophagy protects coelomocyte survival by reducing ROS production

A: Cell survival was determined after transfection with AjBNIP3 siRNA and subsequent LPS exposure for 12 h. *: P<0.05;**: P<0.01,n=3. B: Apoptosis rate was determined using an Annexin V-FITC/PI apoptosis assay kit after transfection with AjBNIP3 siRNA and subsequent LPS exposure for 12 h. *: P<0.05;**: P<0.01,n=3. C: Quantification and immunoblots of AjBNIP3 in coelomocytes after treatment with different concentrations of lactate for 24 h. *: P<0.05;**: P<0.01,n=3. D: Detection of mitophagy flux in coelomocytes after treatment with different concentrations of lactate for 24 h. E: Apoptosis rate was determined using an Annexin V-FITC/PI apoptosis assay kit after treatment with different concentrations of lactate for 24 h. *: P<0.05;**: P<0.01,n=3. F: Survival rate was determined using an MTT kit after treatment with different concentrations of lactate for 24 h. *: P<0.05;**: P<0.01,n=3.