Table 2.
The IC50 (μM) values from dose response curves obtained after treatment of JIMT-1 and MCF-7 breast cancer cells and normal like MCF-10A breast epithelial cells with the SLs isolated from the ethyl acetate fraction of V. leopoldi.
| Cell lines | Vernomenin (1) | Vernolepin (5) | 11ß,13-Dihydrovernodalin (6) | 11ß,13-Dihydrovernodalol (7) |
|---|---|---|---|---|
| JIMT-1 | 2.2 ± 0.9 | 1.7 ± 0.3 | 2.2 ± 0.8 | 1.6 ± 0.05 |
| MCF-7 | 35.0 ± 16.5 | 15.9 ± 0.6 | 4.9 ± 0.3 | 3.9 ± 0.7 |
| MCF-10A | 1.6 ± 0.1 | 2.3 ± 0.2 | 2.0 ± 0.5 | 2.2 ± 0.9 |
The number of 96-well assays run for each compound is found in Fig. 4 as each assay generates one curve. The replicates are ≥ 3 96-well plate independent assays with n = 6 wells in each assay. The data are presented as mean ± SD.