Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 3;54(3):306–317. doi: 10.1038/s41588-022-01014-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — (A) A schematic from the UCSC genome browser representing the Cdkn1b-Lockd gene locus. (B) Relative expression of Lockd upon; siRNA induced knockdown in NIH/3T3 cells (n = 3 biologically independent samples), primary fibroblasts (n = 3 biologically independent samples) and ASO induced knockdown in primary fibroblasts (n = 5 biologically independent samples) measured by RT-qPCR. (C) Scatter plot showing p-values for co-expression of Lockd against genes in proximity for the CAST and C57 genomes (Fisher’s exact test, ± 300 kb of TSS-Lockd, genes filtered for > = 3 allelic counts). The red dashed lines denote threshold for significance (p < 0.05). (D) Scatter plot showing the magnitude (x-axis, SCDE maximum likelihood estimate of the fold change) against significance levels (y-axis, SCDE p-value, two-sided test using the multiple testing corrected (Holm procedure) z-score) for shLockd contrasted shControl treated NIH/3T3 cells. The red dashed line denotes threshold for significance (p < 0.05). Black and grey colored genes represent significant and non-significant fold changes, respectively. (E) Schematic representation for the intersection of fold change (shLockd / shControl) against gene-gene correlations (in shControl treated NIH/3T3 cells). (F) Relative expression of Kif14 upon ASO induced knockdown of Lockd, measured by RT-qPCR (n = 5 biologically independent samples). B, F; data presented as mean values + /- s.e.m., p-values represent a two-sided Student’s t-test.