Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 3;54(3):306–317. doi: 10.1038/s41588-022-01014-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a, Quantification of colony-forming cells on siRNA- and ASO-induced knockdown of Lockd in NIH/3T3 cells. b, Relative expression of Cdkn1b on siRNA-induced knockdown of Lockd in NIH/3T3 cells (left) and primary fibroblasts (right) measured by RT–qPCR. c, Scatterplot representing the magnitudes of fold changes of gene expression (shLockd/shControl) for significant genes (SCDE P < 0.05, two-sided test using the multiple testing-corrected (Holm procedure) z-score) of stably transduced NIH/3T3 cells (x axis) against gene correlations to Lockd in stably shControl-transduced NIH/3T3 cells (y axis). Genes reaching the threshold of Spearman correlations (±0.1, denoted with red dashed lines) were considered for downstream analysis. d, Relative expression of candidate genes on siRNA-induced knockdown of Lockd in NIH/3T3 cells measured by RT–qPCR. e, Relative expression of candidate genes on siRNA-induced knockdown of Lockd in primary fibroblast cells measured by RT–qPCR. a,b,d,e, n = 4 biologically independent samples, data presented as mean values ± s.e.m, P values represent a two-sided Student’s t-test.