FIG. 1.

(A) Time course of change in bacterial numbers of P. aeruginosa 5276 after the indicated time during exposure to each antimicrobial agent. (B) Induction of IL-8 production by the BET-1A cells in response to the aliquots of bacterial culture with each antimicrobial agent. The aliquots of bacterial culture, harvested at the indicated time, were filtered and diluted 1:10 in LHC-9 medium and tested with the BET-1A cells for 24 h. IL-8 levels in the culture supernatants was measured as described above. ●, imipenem; ○, ceftazidime; □, levofloxacin; ▵, gentamicin; ■, control. Data represent the mean ± standard deviation of three experiments. ∗, P < 0.001 (compared with ceftazidime, levofloxacin, gentamicin, and control); ∗∗, P < 0.05 (compared with levofloxacin, gentamicin and control); ∗∗∗, P < 0.01 (compared with control).