FIG. 6.

Neutrophil migration induced by the culture supernatants of the BET-1A cells stimulated with aliquots of bacterial culture containing each antimicrobial agent harvested at the indicated time (A) and the inhibitory effects of antihuman IL-8 IgG on neutrophil migration induced by the culture supernatants of the BET-1A cells stimulated with aliquots of bacterial culture containing imipenem (B). The aliquots of bacterial culture were filtered and diluted 1:10 in LHC-9 medium and then tested with the BET-1A cells for 24 h. The culture supernatants were incubated with anti-IL-8 IgG (100 μg/ml; open columns) or control IgG (100 μg/ml; solid columns) for 1 h at 37°C. Data represent the mean ± standard deviation of three experiments. ∗, P < 0.05 (compared with control IgG by Student’s t test).