RT-PCR master mix
| Reagent | Amount |
|---|---|
| PowerTrack™ SYBR™ Green Master Mix | 6.25 μL |
| Forward primers | 0.25 μL |
| Reverse primers | 0.25 μL |
| cDNA | 1 μL |
| Nuclease-free water | 4.25 μL |
| Total RT PCR volume | 12.00 μL |
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