REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-SOX2 (1:200)	Santa Cruz	Cat# sc-365823, RRID:AB_10842165
Mouse anti-OCT4 (1:200)	Santa Cruz	Cat# sc-5279, RRID:AB_628051
Mouse anti-SSEA4 (1:200)	Abcam	Cat# ab16287, RRID:AB_778073
Rabbit anti-Nanog (1:100)	Abcam	Cat# ab21624, RRID:AB_446437
Mouse anti-NESTIN (1:500)	Santa Cruz	Cat# sc-23927, RRID:AB_627994
Rabbit anti-TUBB3, monoclonal (1:2000)	BioLegend	Cat# 801201, RRID:AB_2313773
Goat anti-CHAT, monoclonal (1:200)	Millipore	Cat# AB144P, RRID:AB_2079751
Mouse anti-HB9, monoclonal (1:500)	DSHB	Cat# 81.5C10-c
Rabbit anti-Islet1 (ISL1), monoclonal (1:250)	Abcam	Cat# ab109517; RRID: AB_10866454
Donkey anti-Mouse IgG (H + L), Alexa Fluor 488 (1:500)	Jackson ImmunoResearch	Cat# 715-545- 150, RRID:AB_2340846
Sheep anti-Mouse IgG (H + L), Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	Cat# 515-515-062, RRID:AB_2340331
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor 488 (1:500)	Jackson ImmunoResearch	Cat# 711-545-152, RRID:AB_2313584
Donkey Anti-Rabbit IgG (H+L), Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	Cat# 711-585- 152, RRID:AB_2340621
Recombinant DNA
pMDLg/pRRE	(Dull et al., 1998)	RRID: Addgene_12251
pRSV-Rev	(Dull et al., 1998)	RRID: Addgene_12253
pMD2.G	(Dull et al., 1998)	RRID: Addgene_12259
pCSC-NGN2-IRES-GFP-T2A-Sox11	(Liu et al., 2016)	RRID: Addgene_90214
pCSC-ISL1-T2A-LHX3	(Liu et al., 2016)	RRID: Addgene_90215
pCSC-Ngn2-IRES-ISL1-T2A-LHX3	(Sepehrimanesh and Ding, 2020)	N/A
Chemicals, peptides, and recombinant proteins
mTeSR1 complete kit (basal medium plus 5× supplement)	STEMCELL Technologies	Cat#85850
Dulbecco's modified Eagle’s medium/nutrient mixture F-12 (DMEM-F12)	Hyclone	Cat#SH30023.02
Dulbecco's Modified Eagle Medium (DMEM)	Gibco	Cat# 11965092
Neurobasal Media	Thermo Fisher Scientific	Cat#21103049
MEM Non-Essential Amino Acids Solution (NEAA) (100×)	Thermo Fisher Scientific	Cat#11140050
Glutamax (100×)	Thermo Fisher Scientific	Cat#35050
N2 supplement (100×)	Thermo Fisher Scientific	Cat#17502-048
B27 supplement (50×)	Thermo Fisher Scientific	Cat#17504-044
Retinoic acid (RA)	MilliporeSigma	Cat#R2625
Valproic acid (VPA)	MilliporeSigma	Cat#99-66-1
2-Mercaptoethanol (BME)	Thermo Fisher Scientific	Cat#21985023
Heat Stable Recombinant Human Basic Fibroblast Growth Factor (bFGF)	Gibco	Cat#PHG0367
Human EGF Recombinant Protein	Gibco	Cat# PHG0311L
Heparin	Thermo Fisher Scientific	Cat#BP252450
bFGF	PeproTech	Cat#100-18B
BDNF (Brain Derived Neurotrophic Factor)	PeproTech	Cat#450-02
GDNF (Glial Derived Neurotrophic Factor)	PeproTech	Cat#450-10
NT3 (Neurotrophic factor 3)	PeproTech	Cat# 450-03
Forskolin (FSK)	MilliporeSigma	Cat# F6886
DMSO, Dimethyl Sulfoxide	Thermo Fisher Scientific	Cat# D1391
KnockOut™ Serum Replacement	Thermo Fisher Scientific	Cat#10828010
Penicillin/streptomycin (Pen-Strep)	Thermo Fisher Scientific	Cat#15140122
Dulbecco (d) PBS (without calcium, magnesium)	Thermo Fisher Scientific	Cat#14190250
Triton X-100	MilliporeSigma	Cat#X100
Accutase (100 mL)	Corning	Cat#25-058-CI
Polyethylenimine (PEI)	Polysciences	Cat# 23966-2
Versene	Thermo Fisher Scientific	Cat#A4239101
Matrigel Matrix	Corning	Cat#356234
Bovine serum albumin (BSA)	Thermo Fisher Scientific	Cat# bp9706
Rho kinase (ROCK) inhibitor (Y-27632), 10 mM	MilliporeSigma	Cat#129830-38-2
TRIzol™ Reagent	Invitrogen™	Cat#15596026
SuperScript™ III First-Strand Synthesis System	Invitrogen™	Cat#18080051
Antifade Mounting Medium	Vector Laboratories	Cat#H-1400-10
PowerTrack™ SYBR Green Master Mix	Thermo Fisher Scientific	Cat#A46012
Experimental models: Organisms/strains
Postnatal day 1 (P1) pups	Charles River	C57BL/6(B6) Mouse Inbred 027
Experimental models: Cell lines
Mice Primary Astrocytes	N/A	N/A
iPSC lines	Coriell Institute for Medical Research	Coriell Cat# GM23476, RRID: CVCL_T841
HEK293T cells	ATCC	ATCC Cat# CRL-3216, RRID: CVCL_0063
Oligonucleotides
See Primer List in Table 1.	(Akter et al., 2021)	See Primer List in Table 1.
Other
6-well plate	Corning	Cat#490007-412
Petri Dishes with Clear Lid	Thermo Fisher Scientific	Cat#FB0875713
Armadillo PCR Plate, 96-well T	Thermo Fisher Scientific	Cat# AB2496
24-well cell culture plates	Thermo Fisher Scientific	Cat#142485
0.22-μm vacuum filter	MilliporeSigma	Cat# SLGP033RB
0.45-μm vacuum filter	MilliporeSigma	Cat# SLHV033RB
15 mL polystyrene Conical tube	Thermo Fisher Scientific	Cat# 14-959-53A
50 mL polystyrene Conical tube	Thermo Fisher Scientific	Cat# 14-432-22