Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Neuron maturation medium
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM: F12 (1:1) | N/A | 24 mL |
| Neurobasal media | N/A | 24 mL |
| Pen/Strep (100×) | 1% | 0.5 mL |
| N2 (100×) | 1× | 0.5 mL |
| B27 (50×) | 1× | 1 mL |
| FSK (20 mM) | 5 mM | 12.5 μL |
| BDNF (20 μg/mL) | 10 ng/mL | 25 μL |
| GDNF (20 μg/mL) | 10 ng/mL | 25 μL |
| NT3 (20 μg/mL) | 10 ng/mL | 25 μL |
| Total | N/A | 50 mL |
