Figure 1.

Dose- and voltage-dependent activation of TRPV3 channel currents by carvacrol and 2-APB.A, whole-cell patch-clamp recordings of TRPV3 currents in response to different concentrations of carvacrol ranging from 0.01 to 3.0 mM with an EC₅₀ of 215.0 ± 47.94 μM (n = 6). B, left panel, representative single-channel current (inside-out patches) traces induced by depolarizing potentials from −80 mV to +80 mV in the presence of carvacrol (300 μM); right panel, the I-V relationship of TRPV3 single-channel currents evoked by carvacrol (gray circles) with a unitary conductance of 182.0 ± 6.4 pS (n = 4) obtained by fitting a linear function. C, left panel, representative single-channel current (inside-out patches) traces in response to depolarizing potentials from −80 mV to +80 mV in the presence of 2-APB; right panel, the I-V relationship of TRPV3 single-channel currents in response to 50 μM 2-APB (black circles) with a unitary conductance of 173.2 ± 5.3 pS (n = 4) obtained by fitting a linear function. All statistical data are expressed as the mean ± SD. 2-APB, 2-aminoethyl diphenylborinate; TRPV3, Transient receptor potential vanilloid 3.