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Validation of CCA-specific ligation methods. (A) Schema for three CCA-specific ligation methods. Dnl: T4 DNA ligase, Rnl2: T4 RNA ligase 2, bio: biotin. (B,C) The method using double-strand oligo and Rnl2 has the best ligation efficiency. (B) SYBR Gold staining and (C) Northern blotting for CCA-specific ligation products (ligated tRNA). The blue arrowheads indicate the bands for pre-tRNAs.
